automated filter paper assay determination cellulase activity



Appl Biochem Biotechnol. 2003 Spring;105 -108:689-703. Automated filter paper assay for determination of cellulase activity. Decker SR(1), Adney WS, Jennings E, Vinzant TB, Himmel ME. Author information: (1)National Bioenergy Center, National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, CO 80401,
Recent advances in HTS equipment have led us to automate this traditionally manually intensive assay. and 8. 1/4 in.8. Although these substrates differ in their physical properties from Whatman #1 filter paper. For commercial broths.FPA to Determine Cellulase Activity 691 examined several cellulose compounds for their
ADNEY. accounting for about half *Author to whom all correspondence and reprint requests should be addressed.00 689 Feature Heading Automated Filter Paper Assay for Determination of Cellulase Activity STEPHEN R. can be reproduced in most laboratories with some effort. an insoluble substrate. Golden.gov Abstract
*Author to whom all correspondence and reprint requests should be addressed. Feature Heading. Automated Filter Paper Assay for Determination of Cellulase Activity. STEPHEN R. DECKER,* WILLIAM S. ADNEY, EDWARD JENNINGS,. TODD B. VINZANT, AND MICHAEL E. HIMMEL. National Bioenergy Center, National
It is always a challenge to determine the total cellulase activity efficiently without reducing accuracy. The most common total cellulase activity assay is the filter paper assay (FPA) established by the International Union of Pure and Applied Chemistry (IUPAC). A new procedure to measure the FPA with microplate-based assay
[6] proposed an attractive method for automated filter paper activity/assay determination, but the results obtained by these authors were not similar to those obtained with the standard method proposed by Mandels et al. [4]. The standard method, performed manually, resulted in a FPA of 38.6 FPU/mL in the commercial.
Cite this. Title. Automated filter paper assay for determination of cellulase activity.(Author abstract)(Report). Also Titled. Automated filter paper assay for determination of cellulase activity. Appears In. Applied Biochemistry and Biotechnology, v.107, no.1-3, 2003 March, p.689(15) (ISSN: 0273-2289). Author. Decker, Stephen
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(2) miniaturized colorimetric assay, (3) automated FPA for the measurement of cellulase. 71 activity, (4) fluorescent ... filter paper assay (FPA) is the key method for analysis of total cellulase activity. In. 81. 1976, the filter ... min, as determined by the dinitrosalicylic acid (DNS) assay (Miller 1959; Wood and Bhat. 96. 1988). 97.
XIAO, Z.; STORMS, R; TSANG, A. Microplate-based filter paper assay to measure total cellulase activity. Biotechnol Bioeng 2004, 88: 832–837. DECKER, S. R.; ADNEY, W. S.; JENNINGS, E., VINZANT, T. B.; HIMMEL, M. E. Automated filter paper assay for determination of cellulase activity. Appl Biochem Biotechnol 2003,

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